Caltrans says vegetation control work will continue along southbound Highway 101 just north of San Luis Obispo throughout the week.
The work will take place on the Cuesta Grade between 9 a.m. and 4 p.m. through Thursday and from 10 a.m. to 3 p.m. on Friday.
One lane of traffic will be closed.
On Monday, the work caused a traffic backup all the way to Atascadero.
Work on the northbound side of the Cuesta Grade took place last week.